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custom-built laserscanning multiphoton microscope  (Laserscanning Europe GmbH)
90
Laserscanning Europe GmbH custom-built laserscanning multiphoton microscope
Custom Built Laserscanning Multiphoton Microscope, supplied by Laserscanning Europe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/two+photon+laserscanning+microscope/pm35025341-59-5-4
Average 90 stars, based on 1 article reviews
custom-built laserscanning multiphoton microscope - by Bioz Stars, 2026-10
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multiphoton laser scanning microscope  (Olympus)
96
Olympus multiphoton laser scanning microscope
Multiphoton Laser Scanning Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/FVMPE-RS+Multiphoton+Laser+Scanning+Microscope/pmc05592697-207-4-10
Average 96 stars, based on 1 article reviews
multiphoton laser scanning microscope - by Bioz Stars, 2026-10
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two photon laserscanning microscope  (Coherent Corp)
96
Coherent Corp two photon laserscanning microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Two Photon Laserscanning Microscope, supplied by Coherent Corp, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/Verdi/10__1523_slash_jneurosci__2210___07__2007-79-7-21
Average 96 stars, based on 1 article reviews
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photon laser scan ning 2p microscope  (Nikon)
99
Nikon photon laser scan ning 2p microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Photon Laser Scan Ning 2p Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/Objectives/pm34767261-79-12-25
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confocal microscopy system  (Laserscanning Europe GmbH)
90
Laserscanning Europe GmbH confocal microscopy system
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Confocal Microscopy System, supplied by Laserscanning Europe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser 50 mw mll-fn473  (CNI Laser)
90
CNI Laser laser 50 mw mll-fn473
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Laser 50 Mw Mll Fn473, supplied by CNI Laser, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/blue+laser+illuminations+mll+fn473/pmc04331623-166-17-25
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two photon laser scanning microscope 2plsm  (Vidrio Technologies)
86
Vidrio Technologies two photon laser scanning microscope 2plsm
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Two Photon Laser Scanning Microscope 2plsm, supplied by Vidrio Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/laser+microscope+photon+scanning+two/10__2139_slash_ssrn__4001945-370-18-34
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laserscanning microscope  (Laserscanning Europe GmbH)
90
Laserscanning Europe GmbH laserscanning microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Laserscanning Microscope, supplied by Laserscanning Europe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/laserscanning+microscope/pm33596051-46-6-7
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two-photon laser-scanning microscope  (Sutter Instrument)
90
Sutter Instrument two-photon laser-scanning microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Two Photon Laser Scanning Microscope, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/two+photon+microscope/10__1523_slash_jneurosci__0930___22__2023-91-8-11
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2p laser-scanning microscope  (Sutter Instrument)
90
Sutter Instrument 2p laser-scanning microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
2p Laser Scanning Microscope, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two photon laser scanning microscope  (Olympus)
99
Olympus two photon laser scanning microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Two Photon Laser Scanning Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/custom-built+upright+laser-scanning+microscope/BX61WI%2FBX51WI+Fixed+Stage+Microscope/pm25739505-331-12-16
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nonlinear laserscanning microscope  (Laserscanning Europe GmbH)
90
Laserscanning Europe GmbH nonlinear laserscanning microscope
Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- <t>resolution</t> <t>two-photon</t> image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.
Nonlinear Laserscanning Microscope, supplied by Laserscanning Europe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- resolution two-photon image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.

Journal: Journal of Neuroscience

Article Title: Spatial Organization of Neuronal Population Responses in Layer 2/3 of Rat Barrel Cortex

doi: 10.1523/jneurosci.2210-07.2007

Figure Lengend Snippet: Figure 1. Sensory-evoked calcium transients in identified E1 barrel column. A, Left, Craniotomy over somatosensory cortex. Middleleft,IntrinsicopticalsignalafterstimulationofE1whiskershowingtheresulting“spot.”Theoutlineofthedarkestpixelsis overlaid in both images indicating the position of E1 barrel column. Middle right, Close-up view of the E1 area from a low- resolution two-photon image. Barrel field outlines obtained from cytochrome c staining are overlaid. Note the pipette tip on the right side. Right, L2/3 neurons (green) and astrocytes (yellow) stained with calcium indicator and sulforhodamine 101, respec- tively,intheE1areaandthesurroundingtissue.AneuronwithintheE1barrelborder(red)wastargetedwithapatchelectrodefor cell-attachedrecording.B,CalciummeasurementfromapopulationofneuronsfromadifferentexperimentthaninA(scalebar, 30 m). Fluorescence traces from five simultaneously recorded PW-related L2/3 neurons showing spontaneous (blue asterisks) and sensory-evoked (red arrowheads) calcium transients. Calcium transients were classified as sensory evoked if the initial fluorescence rise and peak occurred within 200 ms after either the onset (away from resting position) or the offset (return to resting position) of the whisker deflection. Note that not all whisker deflections evoke calcium transients. Yellow bars indicate stimulation periods. An example calcium transient associated with offset stimulation is shown on expanded timescale. C, Simul- taneous cell-attached (CA) recording and fluorescence measurement from the L2/3 neuron from the E1 area shown in B. A spontaneous(blueasterisks)andastimulus-evoked(redarrowhead)spikeandtheirassociatedcalciumtransientsareexpanded in the bottom. The spontaneous spike fell outside the 200 ms time windows used to classify spikes as onset (window a) or offset response (window b). D, Electrically (top, ePSTH) and optically (bottom, oPSTH) determined PSTH for the same neuron and the sametrials.Onlyfivefalsepositivesresultedfor16mincontinuousimagingand231stimuliinthisexperiment.Arrowsdepictbins that contain responses to whisker stimulation.

Article Snippet: Twophoton imaging was performed using a custom-built two-photon laserscanning microscope (excitation wavelength, 870 nm; laser model Mira 900-F laser; Verdi-10 pump; Coherent, Santa Clara, CA).

Techniques: Staining, Transferring, Fluorescence, Whisker Assay